Protocols
Protocols included here are for reference only.
You are responsible to determine their suitableness to your
experiments. Due to the variable nature of cultured cell lines,
performance of some protocols may not be readily repeated when
material other than those from Applied Viromics is used.
Please follow links on the right
for .pdf format of the protocol files.
AAV
Characterization
1. Picogreen assay on AAV genome
copy (GC)
titer
2. ID test on AAV shuttle
plasmids
3. SDS-PAGE and silver-staining
on purified
AAV
4. Endotoxin
assay
5. AAV infectious center assay
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AAV
Application
1. AAV infection of cultured cells
2. In vivo administration of AAV
to mouse by tail vein
injection
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Reporter
Gene Expression
1. Green fluorescent protein (GFP)
2. Red fluorescent protein (AsRed)
3. b-galactosidase (lacZ)
4. Luciferase
(Luc)
5. Chloramphenicol acetyl-transferase
(CAT)
6. Alkaline phosphatase
(AP)
7. Glucuronidase
(GUS)
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Molecular Cloning
1. Construction of AAV shuttle
plasmids
2. Construction of AAV
shuttle
plasmid for siRNA
3. Subcloning gene expression cassette into AAV
shuttle plasmids
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Plasmid Map
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Service Descriptions
1. Introduction of custom AAV production services
>>
2. AAV production deliverables and assay
list
>>
3. Construction of gene expression
cassette
>>
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