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AAV Application
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Protocols
  AAV Characterization
  AAV Application
Reporter Gene Assays
Molecular Cloning
Plasmid Maps
Service Descriptions
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  Reference List
 

 

 

 

Protocols

Protocols included here are for reference only. You are responsible to determine their suitableness to your experiments. Due to the variable nature of cultured cell lines, performance of some protocols may not be readily repeated when material other than those from Applied Viromics is used. 

Please follow links on the right for .pdf format of the protocol files.

 

AAV Characterization                                            

1. Picogreen assay on AAV genome copy (GC) titer                     

2. ID test on AAV shuttle plasmids                                           

3. SDS-PAGE and silver-staining on purified AAV                         

4. Endotoxin assay                                                                

5. AAV infectious center assay                                                

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AAV Application                                                        

1. AAV infection of cultured cells                                              

2. In vivo administration of AAV to mouse by tail vein injection       

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Reporter Gene Expression    

1. Green fluorescent protein (GFP)                                           

2. Red fluorescent protein (AsRed)                                           

3. b-galactosidase (lacZ)                                                       

4. Luciferase (Luc)                                                                   

5. Chloramphenicol acetyl-transferase (CAT)                              

6. Alkaline phosphatase (AP)                                                     

7. Glucuronidase (GUS)                                                           

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Molecular Cloning                                                   

1. Construction of AAV shuttle plasmids                                       

2. Construction of AAV shuttle plasmid for siRNA                            

3. Subcloning gene expression cassette into AAV shuttle plasmids     

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Plasmid Map                                       

1. pAAV-CMV-shuttle 2. pAAV-CAG-shuttle
3. pAAV-CAG-shuttle-WPRE 4. pAAV-ds-CMV-shuttle
5. pAAV-siRNA 6. pAAV-dS-siRNA
   
   

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Service Descriptions                         

1. Introduction of custom AAV production services            >>

2. AAV production deliverables and assay list                    >>

3. Construction of gene expression cassette                     >>

 
  
 
 
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