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Technology at Applied Viromics

Applied Viromics uses its advanced technologies to deliver the best quality viral vectors for your scientific research. Our carefully designed strategies and highly efficient process enable us to save you cost and time. We establish ourselves as reliable provider of viral vectors because of those techniques and our continuous effort to make them better. 

 
For genes pre-packed to viral vectors, We obtain the gene of interest by high fidelity PCR method using either cDNA library or reverse-transcribed mRNA pools from specific tissues. The gene is then fully sequenced to make sure it is 100% correct.

When specifically mentioned, gene modifications such as point mutations or deletions are made for the purpose of generating functional specific moieties. These include forms of constitutively active, dominant negative, or lacking certain protein-protein interaction domains. Such modifications are based upon literatures with established functional outcome, facilitating detailed studies on signaling pathways and protein function validation. Again, any modifications on gene sequences will be confirmed by sequencing. 

For genes provided by our customer in a plasmid, we ask sequence information from our customer, in the format of GB or plain text. Then scientists at Applied Viromics will clone the gene to our shuttle plasmids for vector production (see the shuttle construction section). 

We encourage our customers to order recombinant viral vectors by simply providing the Genbank ID of the gene. We will then clone the gene and prepare the viral vector.

 

 
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