|
|
Viral vector, no matter it is adenovirus, AAV,
retrovirus, or
baculovirus, is first generated by introducing its
shuttle plasmid
into producing cells together with corresponding
helper
plasmid (or viral genome). Applied
Viromics has optimized the viral production
systems to completely avoid using helper virus, thus eliminating any
chance of helper virus contamination.
|
|
|
Toxic genes
Successful
generation of viral vector is built upon our extensive knowledge of
virology and experience in viral production. However, for toxic genes
that kill producer cells, or genes that are found to interfere with viral
replication, recombinant viral vectors cannot be made.
When ordering your viral vector production, please
let us know if you are aware of any toxicity of the gene. Besides communicating
with you what we find during viral production process, we will repeat
once if the first attempt fails. You will be charged 50% of the cost
when viral vector can not be made in two attempts due to previously
unknown toxicity. We have
developed method to control toxic gene expression during viral
production stage, and you have the option to try it. However, the chance of success is case-by-case.
|
