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Technology at Applied
Viromics
Applied Viromics uses its advanced techniques to
delivery the best quality viral vectors for your scientific research.
Our carefully designed strategies and highly efficient process
enable us to save you cost and time. We establish ourselves as
reliable provider of viral vectors because of those techniques and
our continuous effort to make them better.
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At Applied Viromics, quality is never emphasized
enough. Quality control is realized through our well trained
personnel, strict process documentation, and well developed assays.
These highly sensitive assays will ensure our viral vector
products meet the rigid specifications in the field. A Certificate of
Analysis (COA) is attached to every item shipped from Applied
Viromics, containing assay results and specifications. Below is a list of assay
methods we use in our viral vector characterization.
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Assay
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Viral vector type
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Purpose
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Applicable
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Adeno
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AAV
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Retro
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Baculo
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Picogreen
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+
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+
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genome titer
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Purified vector
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Plaque assay
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+
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+(*)
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+
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infectious titer
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Crude & purified
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Silver staining
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+
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+
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Purity
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Purified vector
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RCA assay
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+
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wt Ad contaminant
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Crude & purified(**)
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Gene insert PCR
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+
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+
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identity
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Purified vector
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Endotoxin assay
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+
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+
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+
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+
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endotoxin level
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Crude & purified
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(*) Retrovirus titer is based on numbers
of Neo-resistant clones formed after infection;
(**) Replication in A549 cells is used
for both crude lysate and purified adeno vector, while PCR on E1
sequence is used only for assaying purified vector.
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